I. mIHC/Multiplex Fluorescence Detection ：

Its principle relies on TSA (Tyramide Signal Amplification) technology—an enzyme-catalyzed signal amplification method for immunoassays. The core mechanism involves horseradish peroxidase (HRP) catalyzing the deposition of tyramide derivatives, enabling efficient signal enhancement of target molecules. Through a cyclic staining strategy, it overcomes the target number limitation of traditional IHC/IF, achieving accurate, efficient, and multiplex labeling of multiple target proteins in cell/tissue samples.

II. Applicable Samples:

Paraffin-embedded sections, frozen sections, cells

III. mIHC/Multiplex Fluorescence Detection Protocol

Protocol for Paraffin-Embedded Sections：

1 Slide Baking

2 Deparaffinization and Antigen Retrieval

3 Cooling and Washing

4 Peroxidase Blocking

5 Primary Antibody Incubation

6 Secondary Antibody Incubation

7 Fluorescent Staining

8 Antibody Stripping

9  Cycle: Primary Antibody Incubation → Fluorescent Staining → Antibody Stripping

10 Mounting and Observation

Protocol for Cell Crawling Slides/Frozen Sections：

1 Fixation (Optional), Antigen Retrieval (Optional), Membrane Permeabilization (Optional)

2 Washing

3 Peroxidase Blocking

4 Primary Antibody Incubation

5 Secondary Antibody Incubation

6 Fluorescent Staining

7 Antibody Stripping

8 Cycle: Primary Antibody Incubation → Fluorescent Staining → Antibody Stripping

9 Mounting and Observation