Fss™ Rabbit Monoclonal Antibody : Multidimensional Four-tier Specific Precision Screening System – Rabbit Genetically Engineered Antibodies

Lamarck Fss™ Rabbit Monoclonal Antibody , supported by a Multidimensional Four-tier Specific Precision Screening System, implement a progressive quality control process from the immunization source to antibody purification. This system enables precise regulation of high specificity and high affinity of antibodies, fostering the development of high-quality Rabbit Genetically Engineered Antibodies.

Fss™ Rabbit Monoclonal Antibody Platform

Stage 1: Immunization with Recombinant Protein + Conformational Epitope Peptides

A combination of recombinant protein and conformational epitope peptides is used as the immunogen to precisely activate the rabbit immune system and elicit target-specific antibodies. Serum samples are collected after immunization, and SPR and cell-based assays are applied to validate antibody specificity and reactivity, enabling the selection of rabbits with high-quality immune responses.

Stage 2: Enrichment by Antigen Specificity and Depletion of Non-B Cells

During B-cell screening, magnetic-activated cell sorting (MACS) is used to isolate B cells that are IgG-positive and antigen-positive. This directly enriches the core population of antibody-secreting cells and greatly improves subsequent screening efficiency.

Stage 3: Functional Validation of Single B Cells to Enhance Antibody Specificity

Individual sorted B cells are cultured separately. The specificity of antibodies secreted by single B cells is comprehensively validated using multiple established techniques, including:SPR (Surface Plasmon Resonance)、ELISA (Enzyme-Linked Immunosorbent Assay)、WB (Western Blot)、IF (Immunofluorescence)、IHC (Immunohistochemistry)

Non-specific clones are eliminated to ensure strict target specificity.

Stage 4: Precise Recombinant Antibody Expression and Multimodal Purification

Antibody variable-region genes from selected positive B cells are cloned, recombined, and efficiently expressed in mammalian cells. Recombinant antibodies are fully characterized using a multi-dimensional validation panel including WB, IHC, IF, IP, and more.

Through optimized cell culture, high-efficiency expression, and fine purification, high-purity, high-specificity, high-affinity rabbit recombinant antibodies are obtained.

Antibody Advantages

High Precision & SpecificitySupported by a four-stage multi-dimensional specificity screening system and five-pillar validation strategy, the entire workflow — from immunogen design to final antibody validation — ensures outstanding specificity and affinity.

Superior QualityValidated in endogenous samples and qualified for IP-grade applications. High dilution ratio and high sensitivity enable reliable performance across WB, IHC, IF, IP, ELISA, and other applications.

High PurityProduced via cloning of antibody variable regions from positive B cells, combined with precise recombinant expression and multi-modal purification.

Strong Batch ConsistencyStandardized gene cloning, expression, and purification minimize lot-to-lot variation, ensuring stable performance and excellent experimental reproducibility.

High Scientific RecognitionWidely used in life science research and cited in multiple peer-reviewed publications, providing robust support for scientific studies.