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Cat No: AB0187
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Number
20ul
$108.00
In stock
0
40ul
$200.00
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0
100ul
$416.00
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Main information
Target
Krs-1/2 Phospho Thr183
Reactivity
Human,Mouse
Applications
WB,IHC,IF,ELISA
MW (kDa)
60kD（ Calculated）
60kD（ Observed）
Host Species
Rabbit
Isotype
IgG
Modified
Phospho
Detailed information
Recommended Dilution Ratio
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#RH0011)
Validation Strategies
Specificity
Phospho-Krs-1/2 (T183) Polyclonal Antibody detects endogenous levels of Krs-1/2 protein only when phosphorylated at T183.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):RNtVI
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C, Ship by ice bag)
Concentration
1 mg/ml
MW(Calculated)
60kD
MW(Observed)
60kD
Clonality
Polyclonal
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Antigen & target information
Immunogen:
The antiserum was produced against synthesized peptide derived from human Mst1/2 around the phosphorylation site of Thr183. AA range:149-198
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Gene Name:
STK3/STK4
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Protein Name:
Serine/threonine-protein kinase 3/4
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Alias:
STK3 ;
KRS1 ;
MST2 ;
Serine/threonine-protein kinase 3 ;
Mammalian STE20-like protein kinase 2 ;
MST-2 ;
STE20-like kinase MST2 ;
Serine/threonine-protein kinase Krs-1 ;
STK4 ;
KRS2 ;
MST1 ;
Serine/threonine-protein kinase 4 ;
Mammalian STE20-like prot
KRS1 ;
MST2 ;
Serine/threonine-protein kinase 3 ;
Mammalian STE20-like protein kinase 2 ;
MST-2 ;
STE20-like kinase MST2 ;
Serine/threonine-protein kinase Krs-1 ;
STK4 ;
KRS2 ;
MST1 ;
Serine/threonine-protein kinase 4 ;
Mammalian STE20-like prot
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Database link
Cell localization
Cytoplasm . Nucleus . The caspase-cleaved form cycles between nucleus and cytoplasm (PubMed:19525978, PubMed:11278283). Phosphorylation at Thr-117 leads to inhibition of nuclear translocation (PubMed:19525978). .
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Tissue Expression
Background
serine/threonine kinase 3(STK3) Homo sapiens This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012],
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Function
Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,enzyme regulation:Inhibited by the C-terminal non-catalytic region. Activated by caspase-cleavage. Full activation also requires homodimerization and autophosphorylation of Thr-180, which are inhibited by the proto-oncogene product RAF1.,Function:Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Phosphorylates NKX2-1 (By similarity). Phosphorylates and activates LATS1 and LATS2.,similarity:Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 SARAH domain.,subcellular location:The caspase-cleaved form cycles between nucleus and cytoplasm.,subunit:Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation (By similarity). Interacts with and stabilizes SAV1. Interacts with RAF1, which prevents dimerization and phosphorylation. Interacts with RASSF1, which leads to enzyme activation.,tissue specificity:Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.,
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Research Areas
>> >>MAPK signaling pathway ;
>> >>Hippo signaling pathway ;
>> >>Hippo signaling pathway - multiple species
>> >>Hippo signaling pathway ;
>> >>Hippo signaling pathway - multiple species
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Signal Pathway
Human Diseases >> Tumor-Related >> Pathways in cancer
Human Diseases >> Tumor-Related >> Non-small cell lung cancer
Environmental Information Processing >> Cell Proliferation & Differentiation Regulation >> MAPK signaling pathway
Environmental Information Processing >> Cell Proliferation & Differentiation Regulation >> Ras signaling pathway
Environmental Information Processing >> Cell Proliferation & Differentiation Regulation >> FoxO signaling pathway
References（{{totalcount}}）
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